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What is DNase-seq used for?

What is DNase-seq used for?

DNase-seq (DNase I hypersensitive sites sequencing) is a method in molecular biology used to identify the location of regulatory regions, based on the genome-wide sequencing of regions sensitive to cleavage by DNase I.

What is HI-C assay?

Capture Hi-C is a genome-wide assaying technique to look at chromatin interactions of specific loci using a hybridization-based capture of targeted genomic regions.

What is open chromatin?

Open chromatin regions (OCRs) are special regions of the human genome that can be accessed by DNA regulatory elements. Several studies have reported that a series of OCRs are associated with mechanisms involved in human diseases, such as cancers. Identifying OCRs using ATAC-seq or DNase-seq is often expensive.

Why is Hi-C important?

Hi-C allows the analysis of non-random 3d-genome structures, chromatin domains, of which topologically-associating-domains (TADs) are thought to be the most important ones: ‚ÄúTADs have been shown to be a fundamental component of the 3D organization of the genome in the nuclear space, restraining and facilitating …

How many reads for Hi-C?

This definition sets the resolution of a HiC experiment as the minimum size window which, when used to calculate the genome coverage, leads to 80% of the windows covered by at least 1000 reads [6, 27].

What does Hi-C stand for?

high vitamin content
The name “Hi-C” referred to its high vitamin content.

What is closed chromatin?

Euchromatin (loose or open chromatin) structure is permissible for transcription whereas heterochromatin (tight or closed chromatin) is more compact and refractory to factors that need to gain access to the DNA template.

What does DNase positive mean?

Positive: When DNA is hydrolyzed, methyl green is released turning the medium colorless around the test organism. Negative: If there is no degradation of DNA, the medium remains green.

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