What is the concentration of resazurin in working solution?

The final working concentration for Resazurin is 44 uM and our goal is to prepare a 10X solution for use in cell culture (e.g. for 200 ul of media/cells, we will add 20 ul of the dye).

How much resazurin do I add?

Resazurin Assay Protocol 2. Incubate for desired period of exposure. 3. Add 20 µl resazurin solution to each well.

What is a good cell viability percentage?

between 80-90%
A good cell viability is anywhere between 80-90% in most of the cell lines.

What factors affect cell viability?

Factors studied included temperature, level of dissolved oxygen, nutrient depletion, and waste product accumulation. Growing cells at temperatures 3-9 degrees lower than optimum (37 degrees C) increased viability but monoclonal antibody production was lowered.

How do you increase cell yield?

One approach to increase protein yield is to increase the total number of cells. In order to increase the number of cells, large bioreactors up to 25,000 liters would be used. A second approach is to increase the number of cells in the same volume, effectively increasing viable cell density.

What does resazurin assay measure?

Product overview. Resazurin Assay Kit ab129732 is a fluorometric/colorimetric assay used to measure the metabolic capacity of live cells. The resazurin assay is commonly used to quantify the number of live cells in a sample, and to monitor cell viability / cytotoxicity.

How the viability of cells can be determined?

Cell viability can be calculated using the ratio of total live/total cells (live and dead). Staining also facilitates the visualization of overall cell morphology. NOTE: Trypan Blue has a greater affinity for serum proteins than for cellular protein.

How do you dilute resazurin?

Prepare Resazurin stock solution (100x) by dissolving 0.50 g Resazurin sodium salt into 100 ml 1x PBS. Prepare working solution for Resazurin by 1:100 dilution of the prepared stock solution using 1x PBS.

How is cell viability measured?

How do you interpret cell viability?

Cell viability can be calculated using the ratio of total live/total cells (live and dead). Staining also facilitates the visualization of overall cell morphology.

Can cell viability be more than 100?

Yes indeed, you can get more than 100%. Yes, In case of cellular proliferation we can get higher percentage of cellular viability using calculation of ratio between treated cell and non-treated/standard cell OD values in percentage.