What is the size of MYC?
What is the size of MYC?
The c-Myc gene encodes a polypeptide with a predicted molecular weight of 49 kDa but also demonstrates aberrant electrophoretic mobility in western blotting to manifest an apparent molecular weight of around 62 kDa (p62c-myc) .
Is MYC a protein?
1. C-Myc. C-Myc protein is a member of a family of proteins that regulate cell proliferation and apoptosis. The ability of c-myc to regulate apoptotic cell death results from the coordinated activation of c-Myc and several protein partners (such as Max) that facilitate DNA binding and activate transcription.
How many kDa is MYC?
The Myc tag can be used to detect recombinant proteins in bacteria, yeast, insect and mammalian cell systems. Its short peptide sequence is N-EQKLISEEDL-C and has a size of about 1.2 kDa.
Why is MYC hard Targeting?
Yet, drug development aimed at directly targeting Myc has proved challenging. First, as a transcription factor, Myc lacks a specific active site for small molecules, making it difficult to functionally inhibit its activities using strategies similar to those used for kinases.
What is MYC amplification?
MYC Amplification is an inclusion criterion in 2 clinical trials for non-small cell lung carcinoma, of which 0 are open and 2 are closed. Of the trials that contain MYC Amplification and non-small cell lung carcinoma as inclusion criteria, 1 is phase 1 (0 open) and 1 is phase 1/phase 2 (0 open) .
Where is the MYC protein located?
In the human genome, C-myc is located on chromosome 8 and is believed to regulate expression of 15% of all genes through binding on enhancer box sequences (E-boxes).
What causes MYC amplification?
In ovarian cancer, MYC amplification is correlated with inactivation in the breast and ovarian cancer susceptibility protein (BRCA) and retinoblastoma-associated protein (RB1) pathways ; in endometrial cancer, MYC amplification is associated with low estrogen receptor (ER)/FOXA1 activity and TP53 mutations .
How is MYC activated?
Later, MYC was illustrated to be activated through chromosomal translocation in Burkitt lymphoma (see Conacci-Sorrell et al. 2014). MYC is also commonly activated in tumorigenesis as a consequence of both oncogenic and epigenetic events (Boxer and Dang 2001; Eilers and Eisenman 2008; Dang 2012).
What are Myc proteins?
Myc proteins are transcription factors that activate expression of many pro-proliferative genes through binding enhancer box sequences ( E-boxes) and recruiting histone acetyltransferases (HATs).
Is Myc a promising target for anti-cancer drugs?
Constitutive upregulation of Myc genes have also been observed in carcinoma of the cervix, colon, breast, lung and stomach. Myc is thus viewed as a promising target for anti-cancer drugs.
What does MYC protein interactome profiling reveal about tumorigenesis?
Kalkat M. et al.., MYC protein interactome profiling reveals functionally distinct regions that cooperate to drive tumorigenesis. Mol. Cell 72, 836–848 e7 (2018).
What is the role of Myc stability in gene expression?
MYC stability plays a paradoxical role in regulating gene expression. On one hand, MYC protein levels need to be maintained to allow it to function as a general amplifier of transcription ( 2, 3 ). This is accomplished in normal cells by regulating both MYC transcription and MYC protein stability by phosphorylation at Ser62 ( 16 ).