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What wavelengths do phytoplankton absorb?

What wavelengths do phytoplankton absorb?

Absorption by Phytoplankton. Phytoplankton absorb sun light and use this energy to produce energy rich organic material (photosynthesis). Chlorophylls, present in all phytoplankton cells, will cause 2 dominant peaks in absorption spectra, primary at blue ( 440 nm) and secondary peak in red part of the spectra ( 675 nm) …

What is phytoplankton fluorescence?

This phenomenon is known as chlorophyll fluorescence and represents an optical signal unique to photosynthetic organisms. With respect to phytoplankton exposed to a natural light field, this emission is often referred to as “sun-stimulated,” “solar-induced,” “natural,” or “passive” fluorescence.

What is the difference between the excitation and the emission spectrum?

An emission spectrum describes the wavelengths of the spectrum emitted by an energetic object. The excitation spectrum is a range of light wavelengths that add energy to a fluorochrome, causing it to emit wavelengths of light, the emission spectrum2.

What is the difference between absorption and excitation spectra?

While an excitation spectrum shows the wavelengths of light that a sample will absorb to be able to emit at a specified wavelength, an absorption spectrum shows all of the wavelengths at which light is absorbed by the sample.

How do Phytoplanktons produce visible light?

Answer. Explanation: Individual phytoplankton are tiny, but when they bloom by the billions, the high concentrations of chlorophyll and other light-catching pigments change the way the surface reflects light. In natural-color satellite images (top), phytoplankton appear as colorful swirls.

What pigment has its best light absorption at 440 nm and 670 nm?

Chlorophylls absorb light in the blue (440–475 nm) and red (630–675 nm) regions of the spectrum, while carotenoids and phycobilins do so in the 400–500 nm and 540–650 nm ranges, respectively (Bidigare et al., 1990, Bricaud et al., 2004, Jeffrey and Vesk, 1997).

Why can fluorescence be used to estimate phytoplankton biomass?

The unique wavelength of fluorescence (685 nm) and spectral distance between excitation and emission wavelengths has made fluorescence a reliable proxy for phytoplankton biomass for over 50 years [2]. Historically, fluorescence was primarily measured in vitro.

What is excitation spectra?

Excitation spectra are specific to a single emitting wavelength/species as opposed to an absorbance spectrum, which measures all absorbing species in a solution or sample. The emission and excitation spectra for a given fluorophore are mirror images of each other.

What process does the excitation spectrum correspond to?

The fluorescence excitation spectrum characterizes the electron distribution of the molecule in the ground state. Excitation is equivalent to absorption since upon absorption, the molecule reaches the excited state Sn.

How does phytoplankton get energy?

Phytoplankton make their energy through photosynthesis, the process of using chlorophyll and sunlight to create energy. Like other plants, phytoplankton take in carbon dioxide and release oxygen.

What is the primary method of energy acquisition for phytoplankton?

Phytoplankton obtain energy through the process of photosynthesis and must therefore live in the well-lit surface layer (termed the euphotic zone) of an ocean, sea, lake, or other body of water. Phytoplankton account for about half of all photosynthetic activity on Earth.

Which color has the highest absorbance?

a) The wavelength range that exhibits the greatest absorbance is 600-670 nm, which corresponds to the colors orange and a little red.

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